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KMID : 1005220130280010001
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Journal of Embryo Transfer
2013 Volume.28 No. 1 p.1 ~ p.6
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Optimal Derivation Timing for Establishment of Porcine Embryonic Stem Cells
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Kim Eun-Hye
Cheong Seung-A
Yoon Jun-Chul
Jeon Yu-Byeol
Hyun Sang-Hwan
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Abstract
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A lot of works have been dedicated to clarify the reasons why the establishment of embryonic stem cells (ESCs) from pig is more difficult than that from mouse and human. Several concomitant factors such as culture condition including feeder layer, sensitivity of cell to cell contact, definitive markers of pluripotency for evaluation of the validity and optimal timing of derivation have been suggested as the disturbing factors in the establishment of porcine ESCs Traditionally, attempts to derive stem cells from porcine embryos have depend on protocols established for mouse ESCs using inner cell mass (ICM) for the isolation and culture. And more recently, protocols used for primate ESCs were also applied. However, there is no report for the establishment of porcine ESCs. Indeed, ungulate species including pigs have crucial developmental differences unlike rodents and primates. Here we will review recent studies about issues for establishment of porcine ESCs and discuss the promise and strategies focusing on the timing for derivation and pluripotent state of porcine ESCs.
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KEYWORD
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pigs , embryonic stem cells , early embryo development , pluripotent state
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